The effect of non-invasive dermal electroporation on skin barrier function and skin permeation in combination with different dermal formulations

The aim of this research was to investigate the effect non-invasive dermal electroporation (EP) on barrier function skin and permeation a model macromolecule in combination with different formulations. Skin samples were treated EP treatment for 2 min. Firstly, examined mouse vivo by measuring transepidermal water loss (TEWL). Then, human investigated ex under fluorescence microscope solution, hydrogel, film-forming system (FFS) containing 4 kDa fluorescein isothiocyanate-dextran (FITC-dextran) or without EP. increased intensity shows presence FITC-dextran layers. results showed, that TEWL values rapidly after treatment, it took approximately 5 min be restored. experiments showed just slight could noticed from any formulation EP; however, solution FFS highly decreased reversibly structure SC restored short time treatment. FITC-dextran, as macromolecule, can slightly permeate into passive diffusion. increase rate remarkably compared control treatments; composition formulations has great influence permeation. • reversible function, is enhancer measured microscopy Electroporation